Misty and Isabelle have a strained relationship. Due to certain circumstances Isabelle wasn't really there for much of Misty's life neither was her father. However it is uncertain to the extent their relationship is now as Isabelle was only featured in the finale of Minecraft Royalty for about 8 or so minutes.
Nicholas and Isabelle are former lovers. They had two children, Misty and Evan. Nicholas was shown to still love Isabelle and stated that he needed her, but she snapped and said "You may need me, but I don't need you."
Evan is her son. Evan blames Isabelle for not being there in his life and snapped when she said she was disappointed in him.
She is Julius' Mother. Other than that their relationship is unknown.